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Recombinant Human OSM (N-6His)

Recombinant Human OSM (N-6His)

Recombinant Human OSM (N-6His)

Recombinant Human OSM (N-6His), also known as Oncostatin M, is a multifunctional glycoprotein belonging to the IL-6 cytokine family. It is produced in an E. coli expression system with an N-terminal 6×His tag, corresponding to the Ala26–Arg221 amino acid sequence of human OSM. OSM binds to heterodimeric receptor complexes composed of gp130 and OSMRβ or LIFR, activating multiple signaling pathways including JAK-STAT and MAPK. It exerts a wide range of biological functions, such as inhibiting tumor cell proliferation, inducing cell differentiation, and regulating inflammatory responses. In stem cell research, OSM effectively promotes the directed differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), and participates in key physiological processes including liver development, hematopoiesis regulation and bone metabolism. Manufactured under strict quality control and functionally validated, this product features high purity, high specific activity and low endotoxin, and is suitable for various applications such as cell culture, organoid differentiation, signaling pathway research and drug screening.

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产品介绍

Recombinant Human OSM (N-6His), also known as Oncostatin M, is a multifunctional glycoprotein belonging to the IL-6 cytokine family. It is produced in an E. coli expression system with an N-terminal 6×His tag, corresponding to the Ala26–Arg221 amino acid sequence of human OSM. OSM binds to heterodimeric receptor complexes composed of gp130 and OSMRβ or LIFR, activating multiple signaling pathways including JAK-STAT and MAPK. It exerts a wide range of biological functions, such as inhibiting tumor cell proliferation, inducing cell differentiation, and regulating inflammatory responses. In stem cell research, OSM effectively promotes the directed differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), and participates in key physiological processes including liver development, hematopoiesis regulation and bone metabolism. Manufactured under strict quality control and functionally validated, this product features high purity, high specific activity and low endotoxin, and is suitable for various applications such as cell culture, organoid differentiation, signaling pathway research and drug screening.

培养效果图

Culture icrograph

产品别称

Oncostatin-M; OSM

氨基酸序列

Ala26–Arg221 (Uniprot: P13725)

理论分子量

24.44 kDa

表观分子量

~28 kDa (reduced SDS-PAGE)

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

20 mM Tris-HCl, 1 mM EDTA, 200 mM NaCl, pH 7.5

纯度

≥95% (reduced SDS-PAGE); ≥90% (SEC-HPLC)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 0.2–1 ng/mL (TF-1 cell proliferation assay)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage. Repeated freeze-thaw cycles are strictly prohibited.

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