产品与服务

Recombinant Human KGF

Recombinant Human KGF

Recombinant Human KGF

Recombinant Human KGF (Keratinocyte Growth Factor), also known as FGF-7 (Fibroblast Growth Factor 7), is a secreted growth factor in the FGF family that specifically acts on epithelial cells. It is produced in an E. coli expression system in a tag-free form, corresponding to the Cys32–Thr194 amino acid sequence of human KGF protein. By specifically binding to the FGFR2 IIIb receptor on the surface of epithelial cells, KGF activates downstream signaling pathways including MAPK and PI3K/Akt, and potently promotes the proliferation, migration and differentiation of keratinocytes and various epithelial cells. It also participates in physiological processes such as tissue injury repair and organ morphogenesis. As an important supplement in organoid culture systems, KGF can significantly promote the growth and structural maintenance of epithelial organoids such as intestinal, lung, mammary and skin organoids, and is widely used in epithelial biology research, organoid line establishment, wound repair and drug screening. Manufactured under strict quality control and functional validation, this product features high purity, high specific activity, low endotoxin and excellent batch-to-batch consistency.

规格
货号
NGH030008
价格
￥2313
咨询客服

产品介绍

Recombinant Human KGF (Keratinocyte Growth Factor), also known as FGF-7 (Fibroblast Growth Factor 7), is a secreted growth factor in the FGF family that specifically acts on epithelial cells. It is produced in an E. coli expression system in a tag-free form, corresponding to the Cys32–Thr194 amino acid sequence of human KGF protein. By specifically binding to the FGFR2 IIIb receptor on the surface of epithelial cells, KGF activates downstream signaling pathways including MAPK and PI3K/Akt, and potently promotes the proliferation, migration and differentiation of keratinocytes and various epithelial cells. It also participates in physiological processes such as tissue injury repair and organ morphogenesis. As an important supplement in organoid culture systems, KGF can significantly promote the growth and structural maintenance of epithelial organoids such as intestinal, lung, mammary and skin organoids, and is widely used in epithelial biology research, organoid line establishment, wound repair and drug screening. Manufactured under strict quality control and functional validation, this product features high purity, high specific activity, low endotoxin and excellent batch-to-batch consistency.

培养效果图

Culture icrograph

产品别称

Keratinocyte Growth Factor; FGF-7; Fibroblast Growth Factor 7; HBGF-7

氨基酸序列

Cys32–Thr194 (Uniprot: P21781)

理论分子量

18.9 kDa

表观分子量

~17 kDa (reduced SDS-PAGE)

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

20 mM Tris, 1 mM EDTA, 5% Trehalose, 0.02% Tween 80, pH 8.0

纯度

≥95% (reduced SDS-PAGE)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 0.5–5 ng/mL (BaF3-FGFR2IIIb cell proliferation assay)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage. Repeated freeze-thaw cycles are strictly prohibited.

相关产品