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Recombinant Human FGFa (140AA)

Recombinant Human FGFa (140AA)

Recombinant Human FGFa (140AA)

Recombinant Human FGFa/FGF1 (140AA), also known as recombinant human acidic fibroblast growth factor (FGF-1), is a non-glycosylated heparin-binding growth factor of the FGF family. It is produced in an E. coli expression system in a tag-free form, corresponding to the Phe16–Asp155 amino acid sequence of human FGF1 protein, totaling 140 amino acids. FGFa binds to and activates multiple fibroblast growth factor receptors including FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3c and FGFR4. With the synergistic effect of heparin/heparan sulfate, it activates downstream signaling pathways such as MAPK, PI3K/Akt and PLCγ, and broadly promotes the proliferation, migration and survival of cells derived from mesoderm, neuroectoderm and endoderm. This factor is widely involved in physiological processes including angiogenesis, tissue repair, embryonic development and neuroprotection, and serves as an important basic growth factor in stem cell culture, organoid line establishment and endothelial cell research. Manufactured under strict quality control and functional validation, this product features high purity, high specific activity, low endotoxin and excellent batch-to-batch consistency.

规格
货号
NGH030006
价格
￥763
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产品介绍

Recombinant Human FGFa/FGF1 (140AA), also known as recombinant human acidic fibroblast growth factor (FGF-1), is a non-glycosylated heparin-binding growth factor of the FGF family. It is produced in an E. coli expression system in a tag-free form, corresponding to the Phe16–Asp155 amino acid sequence of human FGF1 protein, totaling 140 amino acids. FGFa binds to and activates multiple fibroblast growth factor receptors including FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3c and FGFR4. With the synergistic effect of heparin/heparan sulfate, it activates downstream signaling pathways such as MAPK, PI3K/Akt and PLCγ, and broadly promotes the proliferation, migration and survival of cells derived from mesoderm, neuroectoderm and endoderm. This factor is widely involved in physiological processes including angiogenesis, tissue repair, embryonic development and neuroprotection, and serves as an important basic growth factor in stem cell culture, organoid line establishment and endothelial cell research. Manufactured under strict quality control and functional validation, this product features high purity, high specific activity, low endotoxin and excellent batch-to-batch consistency.

培养效果图

Culture icrograph

产品别称

Acidic Fibroblast Growth Factor; aFGF; FGF-1; HBGF-1; ECGF

氨基酸序列

Phe16–Asp155 (Uniprot: P05230), 140 amino acids in total

理论分子量

16.0 kDa

表观分子量

~16 kDa (reduced SDS-PAGE)

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

20 mM Mops, 50 mM Na₂SO₄, 1 mM EDTA, pH 6.7

纯度

≥95% (reduced SDS-PAGE)

内毒素

<0.1 EU/μg (LAL assay)

生物活性

ED₅₀ ≤ 0.5 ng/mL (BALB/c 3T3 cell proliferation assay, with heparin synergy)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage.

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