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Recombinant Human EGF

Recombinant Human EGF

Recombinant Human EGF

Recombinant Human EGF (Epidermal Growth Factor), also known as Urogastrone, is a classic secreted growth factor of the EGF family. It is produced in an E. coli expression system in a tag-free form, corresponding to the Asn971–Arg1023 amino acid sequence of human EGF precursor protein, totaling 53 amino acids, with three pairs of intramolecular disulfide bonds maintaining its stable conformation. By specifically binding to the EGFR (ErbB1) tyrosine kinase receptor on the cell membrane, EGF induces receptor dimerization and activates downstream signaling pathways including MAPK, PI3K/Akt and PLCγ, and potently promotes the proliferation, migration and differentiation of epithelial cells, keratinocytes and various mesenchymal cells. It is one of the core basic growth factors in organoid culture systems. It can significantly improve the establishment efficiency, budding capacity and long-term passaging stability of various normal and tumor organoids including intestinal, gastric, hepatic, mammary, pulmonary and cutaneous organoids, and is widely used in stem cell culture, epithelial biology research, wound repair and drug screening. Manufactured under strict quality control and functional validation, this product features high purity, ultra-high specific activity, low endotoxin and excellent batch-to-batch consistency.

规格
货号
NGH030005
价格
￥363
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产品介绍

Recombinant Human EGF (Epidermal Growth Factor), also known as Urogastrone, is a classic secreted growth factor of the EGF family. It is produced in an E. coli expression system in a tag-free form, corresponding to the Asn971–Arg1023 amino acid sequence of human EGF precursor protein, totaling 53 amino acids, with three pairs of intramolecular disulfide bonds maintaining its stable conformation. By specifically binding to the EGFR (ErbB1) tyrosine kinase receptor on the cell membrane, EGF induces receptor dimerization and activates downstream signaling pathways including MAPK, PI3K/Akt and PLCγ, and potently promotes the proliferation, migration and differentiation of epithelial cells, keratinocytes and various mesenchymal cells. It is one of the core basic growth factors in organoid culture systems. It can significantly improve the establishment efficiency, budding capacity and long-term passaging stability of various normal and tumor organoids including intestinal, gastric, hepatic, mammary, pulmonary and cutaneous organoids, and is widely used in stem cell culture, epithelial biology research, wound repair and drug screening. Manufactured under strict quality control and functional validation, this product features high purity, ultra-high specific activity, low endotoxin and excellent batch-to-batch consistency.

培养效果图

Culture icrograph

产品别称

Epidermal Growth Factor; EGF; Urogastrone; Pro-Epidermal Growth Factor

氨基酸序列

Asn971–Arg1023 (Uniprot: P01133), 53 amino acids in total

理论分子量

6.2 kDa

表观分子量

~11 kDa (reduced SDS-PAGE)

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

20 mM Tris, 200 mM NaCl, 4% Mannitol, pH 8.0

纯度

≥95% (reduced SDS-PAGE)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 60–450 pg/mL (BALB/c 3T3 cell proliferation assay)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage. Repeated freeze-thaw cycles are strictly prohibited.

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