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Recombinant Human HGF (C-6His)

Recombinant Human HGF (C-6His)

Recombinant Human HGF (C-6His)

Recombinant Human HGF (C-6His), also known as Scatter Factor (SF), is a multifunctional secreted glycoprotein. Produced in a mammalian cell expression system with a C-terminal 6×His tag, it corresponds to the Gln32–Ser728 amino acid sequence of human HGF protein and naturally forms a heterodimer structure linked by a disulfide bond between the α-chain and β-chain. By specifically binding to the c-Met tyrosine kinase receptor on the cell membrane, HGF activates multiple downstream signaling pathways including PI3K/Akt, MAPK and STAT, and potently regulates cell proliferation, migration, invasion and morphogenesis. It plays a core role in organ development, tissue regeneration, angiogenesis and tumor microenvironment regulation. As a key growth factor in organoid culture systems, HGF can significantly improve the establishment efficiency, budding capacity and long-term expansion ability of various epithelial organoids such as hepatic, intestinal, mammary and lung organoids. Manufactured under strict quality control and functional validation, this product features high purity, high specific activity, low endotoxin and excellent batch-to-batch consistency.

规格
货号
NGH030004
价格
￥1688
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产品介绍

Recombinant Human HGF (C-6His), also known as Scatter Factor (SF), is a multifunctional secreted glycoprotein. Produced in a mammalian cell expression system with a C-terminal 6×His tag, it corresponds to the Gln32–Ser728 amino acid sequence of human HGF protein and naturally forms a heterodimer structure linked by a disulfide bond between the α-chain and β-chain. By specifically binding to the c-Met tyrosine kinase receptor on the cell membrane, HGF activates multiple downstream signaling pathways including PI3K/Akt, MAPK and STAT, and potently regulates cell proliferation, migration, invasion and morphogenesis. It plays a core role in organ development, tissue regeneration, angiogenesis and tumor microenvironment regulation. As a key growth factor in organoid culture systems, HGF can significantly improve the establishment efficiency, budding capacity and long-term expansion ability of various epithelial organoids such as hepatic, intestinal, mammary and lung organoids. Manufactured under strict quality control and functional validation, this product features high purity, high specific activity, low endotoxin and excellent batch-to-batch consistency.

培养效果图

Culture icrograph

产品别称

Hepatocyte Growth Factor; HGF; Scatter Factor; SF; Hepatopoietin-A

氨基酸序列

Gln32–Ser728 (Uniprot: P14210)

理论分子量

81.6 kDa (53.7 kDa α-chain + 27.9 kDa β-chain)

表观分子量

56–65 kDa (α-chain), 30–34 kDa (β-chain) under reduced SDS-PAGE

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

20 mM Tris, 500 mM NaCl, 3% Trehalose, 0.02% Tween 80, pH 8.0

纯度

≥95% (reduced SDS-PAGE)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 0.3–1.5 ng/mL (IL-11 secretion induction assay in Saos-2 cells)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage. Repeated freeze-thaw cycles are strictly prohibited.

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