产品与服务

Wnt3a V3

Wnt3a V3

Recombinant Human Wnt3a V3

Recombinant Human Wnt3a V3 is an engineered Wnt surrogate protein produced in a mammalian cell expression system. By mimicking the function of endogenous Wnt ligands, it specifically binds to the Frizzled (FZD) receptor family and LRP5/6 co-receptors, and potently activates the canonical Wnt/β-catenin signaling pathway. It serves as a core cytokine in organoid culture, stem cell maintenance and differentiation research. Compared with native Wnt3a protein, Wnt3a V3 features superior expression stability, batch-to-batch consistency and bioactivity. It exerts biological functions without lipid modification, addressing industry-wide pain points such as difficult preparation and unstable activity of native Wnt proteins. This product is widely used in the establishment and long-term culture of various organoid models, including intestinal, gastric, hepatic and mammary organoids.

规格
货号
NGH030003
价格
￥1689
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产品介绍

Recombinant Human Wnt3a V3 is an engineered Wnt surrogate protein produced in a mammalian cell expression system. By mimicking the function of endogenous Wnt ligands, it specifically binds to the Frizzled (FZD) receptor family and LRP5/6 co-receptors, and potently activates the canonical Wnt/β-catenin signaling pathway. It serves as a core cytokine in organoid culture, stem cell maintenance and differentiation research. Compared with native Wnt3a protein, Wnt3a V3 features superior expression stability, batch-to-batch consistency and bioactivity. It exerts biological functions without lipid modification, addressing industry-wide pain points such as difficult preparation and unstable activity of native Wnt proteins. This product is widely used in the establishment and long-term culture of various organoid models, including intestinal, gastric, hepatic and mammary organoids.

培养效果图

Culture icrograph

产品别称

Wnt Surrogate-Fc Fusion Protein; Wnt Surrogate

理论分子量

58.5 kDa

表观分子量

66–73 kDa (reduced SDS-PAGE)

缓冲体系

25 mM Tris-HCl, 500 mM NaCl, pH 8.2

剂型

0.2 μm sterile-filtered solution

纯度

≥95% (reduced SDS-PAGE)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 5–25 ng/mL (Topflash reporter gene assay)

保存与运输

Storage: Store at -70°C or below. Valid for 12 months upon receipt. After opening, store at -70°C under sterile conditions for up to 3 months. Shipping: Shipped on dry ice. Precautions: Avoid repeated freeze-thaw cycles. Aliquot into single-use volumes upon receipt and store at -70°C protected from light.

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