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Recombinant Human Noggin

Recombinant Human Noggin

Recombinant Human Noggin

Recombinant Human Noggin is a natural antagonist of the BMP signaling pathway, belonging to the secreted glycoprotein family. It is produced in a mammalian cell expression system in a tag-free form, corresponding to the Gln28–Cys232 amino acid sequence of human Noggin protein. Noggin binds with high affinity to ligands such as BMP-2, BMP-4 and BMP-7, preventing them from binding to BMP receptors on the cell membrane, thereby specifically inhibiting the activation of the BMP/Smad signaling pathway. As one of the core cytokines in organoid culture systems, Noggin effectively maintains the stemness of epithelial stem cells, inhibits spontaneous cell differentiation, and significantly improves the establishment efficiency and long-term passaging stability of various normal and tumor organoids including intestinal, gastric, hepatic, mammary and lung organoids. Manufactured under strict quality control and functional validation, this product features high purity, high bioactivity, low endotoxin and excellent batch-to-batch consistency.

规格
货号
NGH030002
价格
￥1563
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产品介绍

Recombinant Human Noggin is a natural antagonist of the BMP signaling pathway, belonging to the secreted glycoprotein family. It is produced in a mammalian cell expression system in a tag-free form, corresponding to the Gln28–Cys232 amino acid sequence of human Noggin protein. Noggin binds with high affinity to ligands such as BMP-2, BMP-4 and BMP-7, preventing them from binding to BMP receptors on the cell membrane, thereby specifically inhibiting the activation of the BMP/Smad signaling pathway. As one of the core cytokines in organoid culture systems, Noggin effectively maintains the stemness of epithelial stem cells, inhibits spontaneous cell differentiation, and significantly improves the establishment efficiency and long-term passaging stability of various normal and tumor organoids including intestinal, gastric, hepatic, mammary and lung organoids. Manufactured under strict quality control and functional validation, this product features high purity, high bioactivity, low endotoxin and excellent batch-to-batch consistency.

培养效果图

Culture icrograph

产品别称

Noggin; NOG

氨基酸序列

Gln28–Cys232 (Uniprot: Q13253)

理论分子量

23 kDa

表观分子量

25–33 kDa (reduced SDS-PAGE, glycosylated)

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4

纯度

≥95% (reduced SDS-PAGE); ≥95% (SEC-HPLC)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 10–100 ng/mL (inhibition of BMP-4-induced alkaline phosphatase production in ATDC5 cells)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage. Repeated freeze-thaw cycles are strictly prohibited.

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