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Recombinant Human RSPO1 (C-6His)

Recombinant Human RSPO1 (C-6His)

Recombinant Human RSPO1 (C-6His)

Recombinant Human RSPO1 (C-6His) is a secreted glycoprotein of the R-spondin family, produced in a mammalian cell expression system with a C-terminal 6×His tag, corresponding to the Ser21–Ala263 amino acid sequence of human RSPO1. By specifically binding to LGR4/5/6 receptors, RSPO1 inhibits ZNRF3-mediated endocytosis and degradation of Wnt receptors, and synergistically amplifies the canonical Wnt/β-catenin signaling pathway. It is a core cytokine for long-term organoid culture and maintenance of adult stem cell self-renewal, and can significantly improve the establishment efficiency and expansion capacity of epithelial organoids such as intestinal, gastric and hepatic organoids. Functionally validated in multiple systems, this product features high bioactivity, high purity and strong batch stability, meeting various research and industrial application needs including organoid culture, stem cell research and signaling pathway mechanism studies.

规格
货号
NGH030001
价格
￥1563
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产品介绍

Recombinant Human RSPO1 (C-6His) is a secreted glycoprotein of the R-spondin family, produced in a mammalian cell expression system with a C-terminal 6×His tag, corresponding to the Ser21–Ala263 amino acid sequence of human RSPO1. By specifically binding to LGR4/5/6 receptors, RSPO1 inhibits ZNRF3-mediated endocytosis and degradation of Wnt receptors, and synergistically amplifies the canonical Wnt/β-catenin signaling pathway. It is a core cytokine for long-term organoid culture and maintenance of adult stem cell self-renewal, and can significantly improve the establishment efficiency and expansion capacity of epithelial organoids such as intestinal, gastric and hepatic organoids. Functionally validated in multiple systems, this product features high bioactivity, high purity and strong batch stability, meeting various research and industrial application needs including organoid culture, stem cell research and signaling pathway mechanism studies.

培养效果图

Culture icrograph

产品别称

R-spondin 1; CRISTIN3; Roof plate-specific spondin-1

理论分子量

27.8 kDa

表观分子量

~40 kDa (reduced SDS-PAGE, glycosylated)

氨基酸序列

Ser21–Ala263 (Uniprot: Q2MKA7)

剂型

Sterile lyophilized powder (filtered through 0.2 μm membrane)

缓冲体系

PBS, pH 7.4

纯度

≥95% (reduced SDS-PAGE)

内毒素

<0.01 EU/μg (LAL assay)

生物活性

ED₅₀ = 1–10 ng/mL (Topflash reporter gene assay)

保存与运输

Storage: Store at -20°C or below in a dry and dark place. Valid for 24 months upon receipt. After reconstitution, aliquot and store at -70°C for up to 3 months. Shipping: Shipped with blue ice / at ambient temperature (stable lyophilized formulation). Precautions: Avoid repeated freeze-thaw cycles. After reconstitution, it is recommended to aliquot into single-use volumes for low-temperature storage. Repeated freeze-thaw cycles are strictly prohibited.

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